No such athletes currently compete at the highest elite levels of track.
Another set of updates, for athletes with differences in sex development (DSD), will impact 13 athletes, WA President Sebastian Coe said.
They include Semenya, a two-time Olympic champion at 800 metres who has been barred from that event since 2019.
Semenya and others had been able to compete in events outside the restricted range of 400 metres through 1500 metres, but will now have to undergo hormone-suppressing treatment for six months before competing to be eligible.
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Semenya has been trying to compete in longer events. She finished 13th in her qualifying heat at 5,000 meters at world championships last year.
To compete at next year’s Olympics, she would have to undergo hormone-suppressing treatment for six months, something she has said she will never do again, having undergone the treatment a decade ago under previous rules.