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Around 7000 people are expected to take part in the protest, which will begin in Queens Park, continue across the Victoria Bridge and end in Musgrave Park.
The rally will close roads across the Queensland capital.
Protesters initially attempted to close down Brisbane’s Story Bridge for today’s rally, however Queensland’s chief magistrate ruled the protest posed a significant risk to public safety.
Organisers then vowed to take a different route after the court blocked the Story Bridge plan.
The protest is also expected to coincide with the Brisbane Lions home game.
Today’s group was knocked back from being allowed to protest on the Story Bridge earlier this week.
Police took court action to block the demonstration.
They cited concerns about being able to provide enough staff to police the route at short notice, disruption to emergency service routes and access to major hospitals, and increased traffic due to the Brisbane Lions AFL match.
Up to 10,000 people were initially expected to join.
“Peaceful assembly is not unlawful,” Justice for Palestine spokesperson Remah Naji said.
Protesters in Sydney shut down the famous Harbour Bridge on August 3 after a fight against the state government to access the route.
Around 100,000 people joined the pro-Palestine march, which marked the first time protesters had walked across the bridge.