WASHINGTON – The U.S. evacuated 79 staff and families from the U.S. Embassy in Israel on Friday as the conflict between Israel and Iran intensifies and growing numbers of private American citizens seek information on how to leave Israel and Iran.

An internal State Department memo says the military flight, the second known to have occurred this week, left Tel Aviv for Sofia, Bulgaria, where some or all of the passengers were to get a connecting charter flight to Washington.

The document, which was obtained by The Associated Press, also said that more than 6,400 U.S. citizens in Israel had filled out an online form on Friday alone asking for information about when and if the U.S. government would organize evacuation flights. An additional 3,265 people, some of whom may also have competed the form, called an emergency number seeking assistance.

The document estimated that between 300 and 500 people per day could need evacuation assistance should the U.S. decide to offer flights or ships to get Americans out, as the U.S. ambassador to Israel, Mike Huckabee, has said is being considered.

There are some 700,000 Americans in Israel, many of them dual nationals, according to estimates, although the exact number at any given time is unclear because U.S. citizens are not required to notify the embassy if they are there or when they might leave.

Earlier Friday, before the memo was distributed, State Department spokeswoman Tammy Bruce told reporters that more than 25,000 Americans had reached out for information on leaving Israel, the West Bank and Iran.

She told reporters that those people had sought “information and support” and were “seeking guidance” on departing. She would not give a breakdown of where the queries had come from and would not comment on embassy evacuations.

In Iran, the document said that at least 84 U.S. citizens and lawful permanent residents, or Green Card holders, had crossed into neighboring Azerbaijan by land since the conflict began and that an additional 774 had been granted permission to enter as of Friday.

Nearly 200 American citizens and Green Card holders are awaiting permission to travel overland from Iran to neighboring Turkmenistan, it said.