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Gamora
Gamora (Zoe Saldaña) has been killed off once already at the hands of her father Thanos, and while the Gamora we met in Avengers: Endgame, who Quill still pines for, is not the one who died in Avengers: Infinity War, we doubt that Gunn would be sadistic enough to kill her again.
It was revealed at Comic-Con last July that the Gamora who arrives from the past near the conclusion of Endgame has since become the leaders of the Ravagers, with no memory of her relationship with Quill. With the latter still in love with the Gamora he knew, it remains to be seen whether their romance is ultimately rekindled. The trailer does show Gamora back with the Guardians at one point, so it’s possible that she and Quill will reunite, but either way, given her history, we expect Gamora to stick around.
Prediction: Survives
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Groot
Everyone’s favorite walking tree (voiced by Vin Diesel) has died twice already, giving his own life to protect the rest of the Guardians in their very first adventure together and later a victim of the Blip. Like Gamora, the Groot in the movies now is not the same one from the first film, more like his “son,” for lack of a better way to describe an intelligent plant grown from the seeds of another.
This Groot, however, has evolved from adorable little sapling to awkward, petulant teenager, to fierce warrior, his crucial moment of growth coming when he chopped off his own branch to form a handle for Thor’s Stormbreaker in Infinity War.
His shout of “We are Groot!” during an intense shootout in the trailer, a callback to his last words as he died protecting the Guardians in the first movie, might be another Marvel attempt at misdirection. We have no idea of the context for that yet, but we’re confident that this Groot will live on, either continuing as Rocket’s partner or, perhaps, sticking by Quill’s side should Rocket’s life take another path.